SKU: sku-2202
For selection, isolation and cultivation of yeasts and fungi.   SABOURAUD  DEXTROSE  AGAR isused  for  the  cultivation  dermatophytes,  other pathogenic  and nonpathogenic  fungi  and  yeasts.  It  conforms  to  harmonized  USP/EP/JP  requirements.1,2,3Sabouraud Dextrose Agar is a modification of Dextrose Agar described by Sabouraud.4 Mixture of Peptic digest of animal tissue & Pancreatic digest of Casein provides carbon and nitrogen required for the growth of a wide  variety  of  organisms.  The  high  Dextrose  concentration  and  acidic  pH  of  the  formula  permits selectivity  of  fungi.5  Agar  is  incorporated  into  the  media  as  a  solidifying  agent.  George  enhanced Sabouraud Dextrose Agar with the addition of cycloheximide, streptomycin, and penicillin to produce anexcellent medium for the primary isolation of dermatophytes.6 The medium is often used with antibiotics for the isolation of pathogenic fungi from material containing large numbers of other fungi and bacteria. The fungi maintain their typical cultural appearance and thus may be readily identified according to the standard macroscopic characters described by Sabouraud. Aseptically add 0.5gms Cycloheximide, 40,000 units Streptomycin, 20,000 units Penicillin and to each litre of autoclaved, cooled medium. Alternatively, one  may  add  0.4gms  chloramphenicol  and  0.05gms  cycloheximidS  to  each  liter  of  medium  before autoclaving. The medium is usually inhibitory to most non-pathogenic fungi and bacteria by the addition of antibiotics as above. Inoculate Sabouraud Dextrose Agar (TMH 104) medium with a small number (not more than 100 cfu) of the appropriate microorganism and incubate at 20 - 25°C for 48 - 72 hours.
Product Code: 
TMH 104