For selection, isolation and cultivation of yeasts and fungi. SABOURAUD DEXTROSE AGAR isused for the cultivation dermatophytes, other pathogenic and nonpathogenic fungi and yeasts. It conforms to harmonized USP/EP/JP requirements.1,2,3Sabouraud Dextrose Agar is a modification of Dextrose Agar described by Sabouraud.4 Mixture of Peptic digest of animal tissue & Pancreatic digest of Casein provides carbon and nitrogen required for the growth of a wide variety of organisms. The high Dextrose concentration and acidic pH of the formula permits selectivity of fungi.5 Agar is incorporated into the media as a solidifying agent. George enhanced Sabouraud Dextrose Agar with the addition of cycloheximide, streptomycin, and penicillin to produce anexcellent medium for the primary isolation of dermatophytes.6 The medium is often used with antibiotics for the isolation of pathogenic fungi from material containing large numbers of other fungi and bacteria. The fungi maintain their typical cultural appearance and thus may be readily identified according to the standard macroscopic characters described by Sabouraud. Aseptically add 0.5gms Cycloheximide, 40,000 units Streptomycin, 20,000 units Penicillin and to each litre of autoclaved, cooled medium. Alternatively, one may add 0.4gms chloramphenicol and 0.05gms cycloheximidS to each liter of medium before autoclaving. The medium is usually inhibitory to most non-pathogenic fungi and bacteria by the addition of antibiotics as above. Inoculate Sabouraud Dextrose Agar (TMH 104) medium with a small number (not more than 100 cfu) of the appropriate microorganism and incubate at 20 - 25°C for 48 - 72 hours.