Product Code TM 233
PPLO Agar was described by Morton, Smith and Leberman. It was used in a study of the growth requirements of Mycoplasma, along with the identification and cultivation of this organism. Pivotal information regarding Mycoplasma has been documented by Sabin. Hayflick et al have reported the information regarding the cultivation of Mycoplasma. For the cultivation of Mycoplasma, the medium ingredients and all the supplements should be free of any toxic substances even in small amount.
Many Mycoplasma require serum for their good growth and also presence of antibiotic is necessary to prevent the growth of contaminating organisms. Mostly the Mycoplasma species are aerobic or facultative anaerobic but some are microaerophilic. Few are anaerobic saprophytic Mycoplasma which grow best at 22-35?C while pathogenic strains grow at 35?C. Mycoplasma when grow in the agar medium show typical morphology and form colonies below the agar surface and do no grow without serum.
for isolation and cultivation of Mycoplasma species (PPLO)
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