Product Code TM 2391
Staphylococcal food poisoning ranks as one of the most prevalent causes of gastroenteritis worldwide. Staphylococci ferment glucose to produce acid from glucose. Tryptone Soya Broth with 10% sodium chloride and 1% sodium pyruvate is used for enumeration of Staphylococcus aureus in dairy products and is recommended for enumeration by MPN technique.
For MPN Technique: Inoculate 3 tubes of Tryptone Soya Broth w/ 10% NaCl and 1% sodium pyruvate of each test dilution with 1 ml aliquots of decimal dilutions of sample. Incubate at 35-37?C for 48 hours. Following incubation, transfer a loopful from each positive growth tube to Baird-Parker Agar plates. Colonies of S.aureus on Baird Parker Agar are typically circular, smooth, convex, moist, 2-3 mm in diameter, grey-black to jet black and surrounded by an opaque zone.
for enumeration of Staphylococcus aureus in dairy products by MPN technique
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