TOTAL PLATE COUNT AGAR PLATE W/ β-LACTAMASE

Total Plate Count Agar Plate w/?- Lactamase is a medium meant for precise counting of microorganism populations in foods, water, sewage, or environmental samples. The inclusion of ?-lactamase ensures effective neutralization of ?-lactam antibiotics, thereby ensuring proper and accurate counting and no false low counts from samples with antibiotics.The nutrient medium consists of tryptone which provides nitrogen and carbon sources and amino acids and yeast extract, providing vitamin B complex sources to enhance the growth of organisms. Suitable methods of inoculation are streaking, spreading on plates and the pour plate method, according to APHA guidelines.It can also be employed to count microbial populations in controlled environments like in clean rooms. This nutrient medium is gamma-irradiated and triple-layer wrapped to ensure sterility.