Product Code TMV 847
SIM Veg Medium is prepared by replacing animal peptones like Peptic digest of animal tissue, Beef extract and Peptonized iron with vegetable peptones like Veg peptone, veg extract and Veg peptonized iron respectively. This makes the medium free from BSE/TSE risks. SIM Veg Medium is the modification of animal based SIM medium. SIM Veg Medium is used to differentiate enteric bacilli (Salmonella and Shigella) on the same principles as in SIM medium i.e. on basis of sulphide production, indole formation and motility. It is known that Salmonella serotype Paratyphi A and Salmonella serotype Paratyphi B can be distinguished on the basis of H2S (hydrogen sulphide) production using lead acetate as reported by Jordan and Victorso. H2S reacts with Veg peptonized iron to form black precipitate of ferrous sulphide. Motile organisms intensify the H2S reaction. Motile organisms grow away from line of inoculation showing diffused growth while non-motile organisms grow along the stab line. Tryptophan present in Veg peptone is degraded by specific bacteria to produce indole. Indole is detected by the addition of chemical reagents following incubation period. Add 0.2 ml of Kovac?s reagent to the tube and allow to stand for 10 minutes. A pink to red coloured ring indicates a positive indole reaction.
for determination of hydrogen sulphide production, indole formation and motility of enteric bacilli
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