SHEEP BLOOD SOYABEAN CASEIN DIGEST AGAR PLATE

Sheep Blood Soyabean Casein Digest agar is suitable for the cultivation of fastidious aerobic and anaerobic bacteria, the latter of which can be grown either in deep cultures or by incubation under anaerobic conditions. The absence of carbohydrates and reducing sugars in this medium permits the demonstration of hemolytic reactions which is an important differentiating characteristic for bacteria, especially Streptococcus species. TSA with 5% sheep blood is also suitable for performing CAMP test. Group B Streptococci produce a protein like compound called CAMP factor which acts synergistically with beta toxin, produced by some strains of Staphylococcus aureus. The reaction occurs when a streak of beta-lysin producing S. aureus is inoculated perpendicular to a streak of group B Streptococcus resulting in an area of complete lysis in the shape of an arrowhead or crescent.

For cultivation of wide variety of microorganisms and studying haemolytic reactions.