RAPPAPORT VASSILIADIS SALMONELLA ENRICHMENT BROTH (IP)

Rappaport Vassiliadis Salmonella Enrichment Broth (IP) is designed according to the revised formulation by Van Schothorst et al and is recommended for the selective enrichment of Salmonellae from pharmaceutical products. This medium can also be used in direct enrichment of samples containing low inoculum. Present medium is a modification of the Rappaport Vassiliadis Enrichment Broth described by Van Schothorst and Renauld and is recommended by Indian Pharmacopoeia and is in accordance with the harmonized methodology of USP/EP/BP/JP has been found to be superior to other Salmonella selective medias. Addition of magnesium chloride to the medium was reported by Peterz et al. Salmonella species can be isolated from human faeces without pre-enrichment by using this medium. Salmonella generally survive at little high osmotic pressure, grow at slightly low pH and are resistant to malachite green compared to other bacteria. These characteristics are exploited in this medium for selective enrichment of Salmonella.

For selective enrichment of Salmonella species as per Indian pharmacopoeia.