PAGE’S SALINE

Page’s saline is recommended for concentration of bacteria, including Legionella organisms by membrane filtration for detection and enumeration of Legionella.This medium is also recommended by ATCC. When 1.5% Agar is added to Page’s saline it is used for the isolation of Naegleria and Acanthamoeba from tissues and soil samples. For sample processing add the membrane used for filtration of water in a sterile flask containing 5-25ml of Page’s saline then shake vigorously for atleast 2 minutes. The concentrated solution of organisms is plated directly on Buffered Charcoal Yeast Extract Agar. Alternatively, the sample is subjected to heat treatment or acid treatment to reduce non Legionella bacteria. As the growth of Legionella may be inhibited by overgrowth of other bacterial colonies on the membrane, the method is only suitable for waters containing low bacterial counts.

used as a rinsing solution of membranes in water ?ltration for Legionella detection