NUTRIENT AGAR W/TRYPAN BLUE

Nutrient Agar with Trypan blue is recommended for the detection of aerobic endospores from water samples by APHA. Endospores are formed by certain genera of bacteria, commonly found in soil and water samples. Under favorable conditions of growth medium, endospores are readily converted to vegetative cells. The water samples under test is exposed to heat treatment wherein vegetative cells are destroyed and aerobic spores remain unaffected. The water sample is then filtered through membrane filter and placed on Agar plate. Incubate at 35?0.5?C for 24 ? 2 hours. Further incubation upto 5-7 days for intracellular formation of endospores. Count the number colonies as aerobic endospores. The colonies should be catalase positive.

recommended for detection and enumeration of aerobic endospore formers from water samples by membrane ?ltration