Mueller Hinton agar is used for cultivation of Neisseria & for determination of susceptibility of microorganisms to antibiotics. It is formulated by Mueller and Hinton for the primary isolation of Neisseria species. Bauer and Kirby recommended this medium for performing antibiotic susceptibility tests using a single disc of high concentration. It has become the standard medium for antimicrobial susceptibility testing and its performance is in accordance to Clinical and Laboratory Standard Institute (CLSI), formerly NCCLS and complies with requirements of the WHO, FDA and EUCAST.

For determination of susceptibility of microorganisms to antimicrobial agents.