MUELLER HINTON AGAR

Mueller Hinton agar is used for cultivation of Neisseria & for determination of susceptibility of microorganisms to antibiotics. It is formulated by Mueller and Hinton for the primary isolation of Neisseria species. Bauer and Kirby recommended this medium for performing antibiotic susceptibility tests using a single disc of high concentration. It has become the standard medium for antimicrobial susceptibility testing and its performance is in accordance to Clinical and Laboratory Standard Institute (CLSI), formerly NCCLS and complies with requirements of the WHO, FDA and EUCAST. Mueller Hinton Agar has been selected by the CLSI for several reasons: (i) It demonstrates good batch-to-batch reproducibility for susceptible testing, (ii) It is low in sulfonamide, trimethoprim and tetracycline inhibitors, (iii) It supports the growth of most non-fastidious bacterial pathogens and (iv) Many data and much experience regarding its performance have been recorded. WHO Committee on Standardization of Susceptibility Testing has accepted Mueller Hinton Agar for determining the susceptibility of microorganisms because of its reproducibility.

for cultivation of Neisseria and for determination of susceptibility of microorganisms