MILK AGAR W/CETRIMIDE (DOUBLE PACK) (PART – I)

Milk Agar was modified by Brown and Scott for the confirmation of Pseudomonas aeruginosa in swimming pool waters. Swimming pool water is generally chlorinated potable water but it can also be from thermal springs or salt water. Microorganisms of concern are typically those from the body of the bathers, including the orifices. As P. aeruginosa can survive for longer time in water compared to other microorganisms, it is one of the major indicator organisms in the swimming pool. This organism is mainly responsible for ear and eye infection and is very likely to get disseminated in the swimming pool water due to constant contact of ears and eyes with the water. Modified Pseudomonas Selective Agar w/Cetrimide is formulated in accordance with ISO Committee under the specifications ISO 8360-1:1988 for the detection and enumeration of P. aeruginosa from water. Strains of P. aeruginosa are identified by their pigment production i.e. pyocyanin. P. aeruginosa is the only species of Pseudomonas or gram-negative rod known to excrete pyocyanin

for cultivation and enumeration of Pseudomonas aeruginosa in water