Product Code TM 590
Solid media for Mycobacterial cultivation may be egg-based (Lowenstein Jensen Media) or agar-based (Middlebrook Media). Dubos and Middlebrook developed various formulations containing oleic acid and albumin, which protect Mycobacterium from toxic agents, helping for the growth of tubercle bacilli. Middlebrook 7H9 Agar Base developed by Middlebrook and Cohn is used for cultivation of Mycobacteria. This medium can also be used for sensitivity testing of Mycobacteria and for subculturing of stock cultures on addition of Middlebrook OADC Growth Supplement and glycerol.
Mycobacteria are strict aerobes and therefore increased CO2 tension and aerobic conditions must be provided during incubation. Care should be taken while decontamination of the specimen. Also proper specimen collection (sputum and not saliva) should be ensured. Samples should be carefully handled to avoid contamination.
for isolation, cultivation and sensitivity testing of Mycobacterium tuberculosis
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