Product Code TM 2193

  • Description

    Burman substituted bile salts with teepol in Membrane Enriched Teepol Broth, the membrane filtration test medium used to detect coliform organisms in water. M-Lauryl Sulphate Agar is prepared by substituting teepol with sodium lauryl sulphate.
    The water samples are filtered through sterile membrane filter and then placed face upward on agar plates contains M-Lauryl Sulphate Agar. Burman recommended the following incubation temperatures and durations.
    Unchlorinated waters:
    Coliform organisms :4 hours at 30?C followed by 14 hours at 35?C
    Escherichia coli: 4 hours at 30?C followed by 14 hours at 44?C
    Non-chlorinated organisms benefit from 4 hours? incubation at 30?C but chlorinated organisms require 6 hours incubation at 25?C. After incubation, yellow colonies are formed which should be confirmed further.

  • Principle

    for enumeration of Escherichia coli and coliforms in water, using membrane ?lter technique

  • Microorganism

    • Enterobacter aerogenes
    • Escherichia coli
  • Industry

    • NA
  • Regulation

    • NA
  • Pack Size

    • 500 gm
  • Downloads

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