LURIA BERTANI BROTH, MILLER (MILLER LURIA BERTANI BROTH)

Luria Bertani Broth, Miller is slightly different with double amount of sodium chloride as compared to original media described by Lennox for cultivation and maintenance of recombinant strains of Escherichia coli. The media is nutritionally rich for the growth of pure cultures of recombinant strains. Strains derived from Escherichia coli K12 are deficient in Vitamin B synthesis which are further modified by specific mutation to create auxotrophic strains and are therefore unable to grow on nutritionally deficient media.

for cultivation and maintenance of recombinant strains of Escherichia coli for genetic and molecular studies and may be used for routine cultivation of not particularly fastidious microorganisms