LURIA BERTANI AGAR PLATE

Luria Bertani Agar is prepared as described by Lennox for cultivation and maintenance of recombinant strains of Escherichia coli. Luria Bertani Agar, Miller is slightly different with double amount of sodium chloride. The media is nutritionally rich for the growth of pure cultures of recombinant strains. Strains derived from Escherichia coli K12 are deficient in Vitamin B synthesis are further modified by specific mutation to create auxotrophic strains and are therefore unable to grow on nutritionally deficient media.

For the cultivation and maintenance of recombinant strains of E.coli for genetic and molecular studies, may be used for routine isolation, cultivation of not particularly fastidious microorganisms.