LURIA BERTANI AGAR, MILLER (MILLER LURIA BERTANI AGAR)

Luria Bertani Agar is prepared as described by Lennox for cultivation and maintenance of recombinant strains of Escherichia coli. Luria Bertani Agar, Miller is slightly different with double amount of sodium chloride. The media is nutritionally rich for the growth of pure cultures of recombinant strains. Strains derived from Escherichia coli K12 are deficient in Vitamin B synthesis are further modified by specific mutation to create auxotrophic strains and are therefore unable to grow on nutritionally deficient media.

for cultivation and maintenance of recombinant strains of Escherichia coli