LACTOSE PEPTONE BROTH

Coliform bacteria are commonly used as bacterial indicator of sanitary quality of foods and water. It is a valuable bacterial indicator for determining the extent of faecal contamination of recreational surface waters or drinking water. Coliforms are defined as rod-shaped gram-negative organisms, which ferment lactose with the production of acid and gas when incubated at 35?C.
Lactose Peptone Broth was originally described in German Standard Methods and German Drinking Water Regulations as a non-selective enrichment and detection medium for Escherichia coli and other coliforms in water specimens. German standards suggest the use of MPN technique with 0.1, 1.0 and 10 ml of sample and an incubation at 36 ? 1?C for 44 ? 4 hours. Depending upon the sample volume the medium can be either used as single strength or triple strength. Tubes that change to yellow and eventual gas production in Durham?s tubes are considered positive.

for detection of coliform organisms in water