HEART INFUSION BROTH (BEEF HEART INFUSION BROTH)

Fastidious organisms having exacting nutritional requirement could be cultivated on infusion media, as demonstrated by Huntoon. A liquid medium containing an infusion of meat was one of the first media used for the cultivation of bacteria. These infusion media need not be further supplemented by the addition of supplements for cultivation of fastidious bacteria. Heart Infusion Broth, containing infusion from beef heart is used for the isolation and cultivation of a wide variety of fastidious organisms. Heart infusion media can also be used for the cultivation of Vibrio species. Heart Infusion Broth can also be supplemented with glucose, horse serum and antibiotics for the cultivation a wide variety of organisms. Heart Infusion Broth can be used as a base to study carbohydrate fermentation. This medium was used for isolation and enumeration of haemolytic Streptococci in milk.

for isolation and cultivation of various fastidious microorganisms