FNA MEDIUM (FLUORESCEIN DENITRIFICATION AGAR)

FNA Agar is based on the formula described by Pickett and Pedersen. Fluorescence-Denitrification (FN) Media is formulated to detect fluorescein pigment and complete reduction of nitrate to nitrogen gas. These two characteristics are important in the identification of the pseudomonads and other non-fermentative bacilli. Pseudomonas species may represent a minority of the total microflora at the beginning of shelf life. However, under certain conditions, their capacity for rapid growth decides their dominance. A problem associated with the use of media developed for isolation of Pseudomonas species from foods is the considerable interference from non-pseudomonads.

for di?erentiation of Pseudomonas from other bacilli based on their ability to reduce nitrates or nitrites to nitrogen gas (denitri?cation) and detection of ?uorescein pigment