Product Code TM 1422
Vibrio species are natural inhabitants of brackish and salt water. Human disease is associated with ingestion of contaminated water or consumption of contaminated seafood. Wound and systemic infections develop following contact with contaminated water. CPC (Cellobiose, Polymyxin and Colistin) Agar Base formulated as per APHA is recommended for the cultivation and identification of Vibrio species from foods. CPC Agar is a selective and differential agar medium, designed to differentiate Vibrio vulnificus from other Vibrios. Vibrio cholerae strains except V. cholerae 01-classical biotype grow on CPC Agar while most Vibrio parahaemolyticus strains do not grow on CPC Agar.
Blend approximately 25 grams of food sample with 225 ml Alkaline Peptone Water. Transfer a loopful from the surface growth of either Alkaline Peptone Water or Gelatin Phosphate Salt Broth to the surface of the dried plates of CPC Agar. Streak in a manner that will yield isolated colonies. Incubate CPC Agar at 40 – 42?C for 18 to 24 hours. Typical colonies of V. cholerae on CPC Agar are small, smooth, opaque and green to purple in colour as CPC Agar contains two pH indicators viz. bromothymol blue and cresol red. A purple background will also develop in the CPC Agar upon extended incubation.
for cultivation of Vibrio species from foods
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