Product Code TM 1158
Campylobacter species were associated with variety of veterinary diseases and also has been characterized as bacterial agents of human foodborne gastroenteritis. The organisms may also be transmitted by contaminated food or water. Campylobacter Cefex Agar Base is used for isolation and cultivation of Campylobacter species. Campylobacter Agar with antimicrobics and 50 ml sheep blood is recommended as a selective medium for the primary isolation and cultivation of Campylobacter species. Campylobacter Cefex Agar Base is a highly nutritious base and the addition of horse blood supplements the medium with X-factor and other growth factor requirements.
The addition of antimicrobials to the medium is required to suppress the growth of normal flora. Cefoperazone is added to inhibit many gram-positive and gram-negative organisms (Aerobic and anaerobic). Cycloheximide is added to inhibit the growth of contaminating fungi. Campylobacter Cefex Agar Base can be used for direct inoculation or indirect inoculation. After inoculation, incubate the plates at 42?C for 48-72 hours in microaerophilic atmosphere. In addition, media may be set up in duplicate with the second set incubated at 35-37?C to allow for the growth of certain Campylobacter species. Campylobacter jejuni colony morphology may appear as small mucoid, grayish flat colonies with irregular edges and no hemolytic patterns after 24-48 hours. Colonies may also appear pink or yellowish gray with some colonies exhibiting a tailing effect along the streak line. They may also appear as round, convex, entire, glistening, 1-2 mm in diameter.
Cephalothin-sensitive Campylobacter species such as C.fetus and C.upsaliensis may not be recovered on Campylobacter Cefex Agar Base because it contains cefoperazone. These agents in selective media may inhibit some strains of desired species. Therefore, specimens cultured on selective media should also be cultured on non-selective media to obtain additional information and to ensure recovery of potential pathogens.
for isolation and cultivation of Campylobacter species
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