C.L.E.D. AGAR (W/ BROMO THYMOL BLUE) (BROLACIN AGAR)

Product Code TM 405

  • Description

    On a solid medium, Sandys reported that swarming of Proteus species can be controlled by restricting the electrolytes. Formerly swarming of Proteus was controlled by adding alcohol, surface-active agent, sodium azide, boric acid etc. to the medium. Later on Sandy’s medium was modified by Mackey and Sandys, by replacing mannitol by lactose and sucrose and elevating concentration of agar and bromothymol blue. This formulation was further modified by the same authors, called C.L.E.D. (Cystine-Lactose-Electrolyte-Deficient) by deleting the sucrose and by including L-cystine for promoting the growth of cystine dependent dwarf colony coliforms. This medium is recommended for use in urinary bacteriology, promoting the growth of all urinary pathogens. C.L.E.D. Medium is also recommended for dip stick procedures and as dip inoculum transport medium for urine specimens.
    Bacteriuria may be quantitated by inoculating the surface of an agar medium by proper dilution. Inoculate the medium immediately after urine collection. It can also be inoculated by calibrated loop or duplicate dilution pour plate methods.

  • Principle

    for isolation and differentiation of urinary pathogens by lactose fermentation

  • Microorganism

    • Enterococcus faecalis
    • Escherichia coli
    • Klebsiella pneumoniae
    • Proteus vulgaris
    • Salmonella typhi
    • Staphylococcus aureus subsp. aureus
  • Industry

    • Clinical Diagnostics
  • Regulation

    • NA
  • Pack Size

    • 100 gm
    • 500 gm
  • Downloads

Follow Us

Kindly Follow Us