Product Code TM 364
Salmonella species cause many types of infections, from mild self-limiting gastroenteritis to life threatening typhoid fever. The most common form of Salmonella disease is self-limiting gastroenteritis with fever lasting less than 2 days and diarrhoea lasting less than 7 days. Brilliant Green Agar Base, Modified, as a primary plating medium for isolation of Salmonella species was first described by Kristensen et. al. and further modified by Kauffmann. Brilliant Green Agar is also recommended by APHA, FDA and described in EP, BP and IP.
Clinical specimens can be directly plated on this medium. However, being highly selective, it is recommended that this medium should be used along with a less inhibitory medium to increase the chances of recovery. Often cultures enriched in Selenite or Tetrathionate Broth is plated on Brilliant Green Agar along with Bismuth Sulphite Agar, SS Agar, MacConkey Agar. Brilliant green helps to inhibit the contaminating microflora. The medium can further supplemented with sulphaacetamide (1g/l) and sodium mandelate (0.25g/l) to inhibit contaminating microorganisms when the sample is suspected to contain large number of competing organisms along with Salmonella species. Non-lactose fermenting bacteria develop white to pinkish red colonies within 18 – 24 hours of incubation.
for selective isolation of Salmonellae other than Salmonella Typhi from faeces and foods etc
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