BRAIN HEART INFUSION BROTH

Brain Heart Infusion Broth is useful for cultivating a wide variety of microorganisms since it is a highly nutritive medium. It is also used to prepare the inocula for antimicrobial susceptibility testing. Brain Heart Infusion Broth is a modification of the original formulation of Rosenow, where he added pieces of brain tissues to dextrose broth. BHI Broth is also the preferred medium for anaerobic bacteria, yeasts and molds. It is a nutritious, well buffered medium which supports the growth of a wide variety of organisms. With 10% defibrinated sheep blood addition, it can be used to isolate and cultivate Histoplasma capsulatum and other fungi. For selective isolation of fungi, addition of gentamicin and/or chloramphenicol is recommended. Proteose peptone, Calf brain infusion powder and BHI powder serve as sources of carbon, nitrogen, essential growth factors, amino acids and vitamins. Dextrose serves as a source of energy. Disodium phosphate helps in maintaining the buffering action of the medium whereas sodium chloride maintains the osmotic equilibrium of the medium.

Brain Heart Infusion Broth is the recommended base medium of standard method for the isolation of particular organisms such as Staphylococcus aureus, making it suitable for food and dairy testing applications. Brain Heart Infusion Broth could be customized with selective additives according to the test needs. It can be used to produce a consistent inoculum for reliable inoculum preparation for confirmatory tests under controlled conditions.

for cultivation of fastidious microorganisms associated with blood culture