Product Code TM 1140
Decarboxylase Media used for the detection of arginine dihydrolase and lysine and ornithine decarboxylase was first introduced by Moeller. Arginine Dihydrolase Broth is used for detection of arginine dihydrolase producing microorganisms. These types of media are used to differentiate bacteria on the basis of their decarboxylating activity towards the amino acids. Arginine decarboxylase enzyme is also known as Arginine dihydrolase. Arginine decarboxylase (or dihydrolase) production by various members of enteric bacteria aids in differentiating bacteria with closely related physiological characteristics. Bacteria producing arginine dihydrolase enzyme decarboxylate arginine present in this medium to putrescine. The production of amine, putrescine, elevates the pH. Bromo cresol purple is the pH indicator which forms purple colour in alkaline condition. Colour change from purple to yellow and then back to purple is positive reaction. In differentiation of Enterobacteriaceae, control tubes without arginine must be used. If the tubes give positive purple reaction the test is considered as negative.
for detection of Arginine diffydrolase producing microorganisms
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