ANTIBIOTIC ASSAY MEDIUM NO. 37 (SOYA CASEIN DIGEST MEDIUM) (TRYPTONE SOYA BROTH) (VEG.)

Antibiotic Veg Assay Medium No. 37 is prepared by incorporating vegetable peptones in place of animal peptones, making the medium BSE-TSE risks free. Grove and Randall have elucidated the antibiotic assays and media in their comprehensive treatise on antibiotic assays and are also formulated as per CFR and USP.
Antibiotic Veg Assay Medium No. 37 can be used for the same purpose of Antibiotic Assay Medium No. 37. Hence Antibiotic Veg Assay Medium No. 37 can also be used for cultivation of fastidious and non-fastidious organisms and sterility testing of pharmaceutical preparations.
It is also used for the sensitivity testing by the tube dilution method for antimicrobial agents. Turbidimetric antibiotic assay is based on the change or inhibition of growth of test microorganisms in a liquid medium containing a uniform concentration of an antibiotic. After incubation of the test organism in the working dilutions of the antibiotics, the amount of growth is determined by measuring the light transmittance using spectrophotometer. The concentration of antibiotic is determined by comparing amounts of growth obtained with that given by the reference standard solutions. Use of this method is appropriate only when test samples are clear.

for sterility testing and cultivation of fastidious and non-fastidious microorganisms