ANTIBIOTIC ASSAY MEDIUM NO. 36

The composition of this medium is in accordance to CFR. This medium is recommended for sterility testing. Antibiotic Assay Medium No. 36 is widely employed as seed agar for agar diffusion assay for antibiotic bleomycin. The test organism Mycobacterium smegmatis is also maintained in this medium. This medium is employed for cultivation and isolation of fastidious or non-fastidious microorganisms. This medium is also used as maintenance medium of Psuedomonas aeruginosa for plate assay of ticarcillin. The medium like the conventional medium is used for a multitude of purposes including maintenance of stock cultures, plate counting, isolation of microorganisms from a variety of specimen types and a base for media containing blood.
Freshly prepared plates should be used for antibiotic assays. Test organisms are inoculated in sterile seed agar pre-cooled to 40-45?C and spread evenly over the surface of solidified base agar. All conditions in the microbiological assay must be controlled carefully. The use of standard culture media in the test is one of the important step for the good results.

a general purpose medium for cultivation of a wide variety of fastidious microorganisms