ANTIBIOTIC ASSAY MEDIUM NO. 11 (as per USP)

This medium is formulated in accordance to USP and CFR; and is employed to analyze the neomycin content as per FDA and the USP. It is indentical numerically with the name assigned by Grove and Randall. This medium provides a pH range of 8.3 while Antibiotic assay medium no.1 provides pH range of 6.5-6.7.
Freshly prepared plates should be used for antibiotic assays. Test organisms are inoculated in sterile seed agar pre-cooled to 40-45?C and spread evenly over the surface of solidified base agar. All conditions in the microbiological assay must be controlled carefully.

for microbiological assay of antibiotics