ANTIBIOTIC ASSAY MEDIUM B (as per IP)

This medium is commonly used as base agar for microbiological agar diffusion assays for wide variety of antibiotics. These agar diffusion assays can be performed by cylinders, punched-hole or paper disc tests. This medium is prepared according to the specifications detailed in the IP and CFR.
To perform the antibiotic assay, the Base Agar should be prepared on the same day as the test. For the cylinder method, a base layer of 21 ml is required. Once the base medium has solidified, seed layer inoculated with the standardized culture can be overlaid. Even distribution of the layer is important.

for microbiological assay of antibiotics