AGAR MEDIUM F (CRYSTAL VIOLET, NEUTRAL RED, BILE AGAR W/ GLUCOSE) (as per EP/BP)

It is selective medium recommended for detection of Enterobacteriaceae species as recommended by British Pharmacopoeia and is also cited as Agar medium, F. Mossel et al added glucose to the medium observing improved detection of coliforms. Incubation can be carried out at different temperatures and incubation time depending upon the group of Enterobacteriaceae to be recovered. The red colour is due to absorption of neutral red and a subsequent colour change of the dye when the pH of medium falls below 6.8.

for detection and enumeration of Enterobacteria