ACTINOMYCETE ISOLATION AGAR

Actinomycetes are gram-positive bacteria, which show marked chemical and morphological diversity but form a distinct evolutionary line of organisms that range from coccoid and pleomorphic forms to branched filaments. Actinomycetes form an integral part of soil, water and vegetation. Actinomycete development leads to the formation of volatile metabolites. Traces of these volatile metabolites are sufficient to impart disagreeable odour to water or a muddy flavour to fish. Actinomycetes also cause disruptions in wastewater treatment by forming massive growths, which are capable of producing thick foam in the activated sludge process. Actinomyces Isolation Agar used for isolation and propagation of Actinomycetes from soil and water was formulated by Olsen.
Inoculate the plates with 1 drop of diluted culture or specimen and spread over the surface using a sterile bent glass rod. Incubate at 35-37?C for 40-72 hours. The media can be used for long term storage after sufficient growth is obtained. Agar slants are used for maintenance of cultures over a shorter period of time.

for isolation and propagation of Actinomycetes from soil and water