SKU: SKU-TM1851
Description: 
For the detection of urease production, particularly by members of the genus Proteus. Urea Broth Base is a liquid modification of Christensen medium . The modification is suitable for detection of urease production, particularly by members of the genus Proteus. This medium is especially recommended for the differentiation of Proteus species from Salmonella and Shigella species, based on urea utilization. Urea Broth Base (Christensen) contains peptic digest of animal tissues which act as rich source of essential nutrients. Dextrose is the energy source. Sodium chloride maintains the osmotic equilibrium of the medium whereas phosphates serve to buffer the medium. Urea is hydrolyzed to liberate ammonia. Phenol red indicator detects the alkalinity generated by visible colour change from orange to pink. Prolonged incubation may cause alkaline reaction in the medium. A medium without urea serves as negative control to rule out false positive results. Also, all urea test media rely on the alkalinity formation and so they are not specific for determining the absolute rate of urease activity. The utilization of proteins may raise the pH to alkalinity due to protein hydrolysis and excess of amino acids liberation results in false positive reaction
Product Code: 
TM 1851
Technical Data: 

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