Sabouraud Chloramphenicol Agar is used for the propagation of yeast and molds, particularly the parasitic fungi concerned with skin and scalp lesions. Sabouraud Chloramphenicol Agar was formulated by Scientist “Sabouraud”. The medium contains Mycological peptone which provides nitrogen, vitamins, minerals, amino acids and growth factors. Dextrose serves as the energy and carbon source for fungi. Chloramphenicol inhibits a wide range of gram-positive and gram-negative bacteria which makes the medium selective for fungi. Agar is a solidifying agent. The low pH favors fungal growth and inhibits contaminating bacteria from clinical specimens. For isolation of fungi from contaminated specimens, a selective medium should be inoculated simultaneously.