CETRIMIDE AGAR BASE (VEG.)

SKU: sku-1019
Description: 
For selective isolation of Pseudomonas aeruginosa from clinical samples. CETRIMIDE  AGAR  BASE  (VEG.) isused  for  the  selective  isolation  of Pseudomonas  aeruginosa.  The medium  contains  quaternary  ammonium,  cationic  detergent  compound  like  Cetrimide  which  has  a property to inhibit all the bacteria except P. aeruginosa thus making the medium selective for Pseudomonasaeruginosa.  Veg.  gelatin  digest  provide  the  nitrogen  and  carbon  sources.  Cetrimide  causes  release  of nitrogen  and  phosphorus  from  bacterial  cells  other than P.  aeruginosa.  Agar  is  solidifying  agent. Magnesium chloride and potassium sulphate stimulates the bacterium to produce and secrete a pigment called pyocyanin due to which the colony colour turns light green, and also they forms a grape like odour due  to  production  of  aminoacetophenone.  These  are  the  typical  characteristics  that  help  isolating P.aeruginosa using cetrimide agar base. On incubation of plates in an inverted position (agar side up) at 35 ± 2°C for 18-48 hours of P. aeruginosa showed profuse growth whereas strain of E.coli inhibits the growth in this medium. If the cfu/ml is high then the test sample can be directly inoculated onto this cetrimide medium.
Product Code: 
TMV 060