SOYBEAN CASEIN DIGEST MEDIUM (TRYPTONE SOYA BROTH) (CASO BROTH) (as per USP/EP/JP/BP)

Soybean casein digest medium (as per USP/EP/JP/BP/IP) is used for cultivation of microorganism and sterility testing of molds and bacteria. This medium is used for the sensitivity testing of antimicrobial agents by the tube dilution method. It is also employed in diagnostic research in microbiology. This medium is used as a diluent and suspending medium for preparation of samples or test strains. It is also employed in sample preparation for testing of products, wherein incubation is carried out, only to serve sufficient resuscitation of the cell, while avoiding multiplication of the organism.

Soybean Casein Digest Medium is a popular general purpose medium for culturing a variety of microorganisms. It is particularly suitable for testing the sterility of molds and bacteria, making it a reliable choice in pharmaceutical and microbiology laboratories. Soybean Casein Digest Medium is based on harmonized monographs of major pharmacopoeias including USP, EP, BP, JP and IP and is provided as a solution for use in the regulated environment. Soybean Casein Digest Medium has a nutritious environment, as it contains digests of soybean meal and casein. These two ingredients contain essential amino acids and peptides which can be used by both fastidious and non-fastidious organisms. The natural sugars from the soybean also promote the growth of microbes, as well as the sodium chloride maintains the osmotic balance. Dipotassium hydrogen phosphate is employed as a buffer agent to maintain the pH in a stable state and glucose as a fermentable source of energy. This broth is widely utilized in the sterility test, microbial limit test and antimicrobial activity test. Soybean Casein Digest Medium is also used as a diluent and as a suspending medium in sample preparation, especially when recovery and resuscitation of microorganisms is needed without excessive growth. Depending on the laboratory requirements, it can be prepared as a solid or liquid. Soybean Casein Digest Medium Preparation is easy and convenient. Dissolve the powder in distilled water, heat on a water bath and sterilize by autoclaving. The outcome is a transparent light yellow solution with a stable pH that ensures consistent performance. For agar applications, it can be poured into plates after it cools. Soybean Casein Digest Medium is subjected to quality and performance testing through growth promotion studies, for the reliable recovery of the microorganism under specified incubation conditions. It permits the growth of bacteria in 18-24 hours and fungal growth within a few days.

for cultivation of wide variety of microorganisms recommended for sterility testing of moulds and lower bacteria