LACTOBACILLUS MRS AGAR (MRS AGAR)

Lactobacilli MRS medium is based on the formulation of deMan, Rogosa and Sharpe with slight modification. It supports luxuriant growth of all Lactobacilli from oral cavity, dairy products, foods, faeces and other sources. Lactobacilli are microaerophilic and generally require layer plates for aerobic cultivation on solid media. When the medium is set, another layer of un-inoculated Lactobacilli MRS Agar is poured over the surface to produce a layer plate. Lactobacilli isolated on MRS Agar should be further confirmed biochemically. Lactobacilli MRS medium consists of proteose peptone and beef extract which supply nitrogenous and carbonaceous compounds. Yeast extract provides vitamin B complex and dextrose is the fermentable carbohydrate and energy source. Polysorbate 80 supplies fatty acids required for the metabolism of Lactobacilli. Sodium acetate and ammonium citrate inhibit Streptococci, molds and many other microorganisms. Magnesium sulphate and manganese sulphate provide essential ions for multiplication of lactobacilli. Phosphates provide good buffering action in the media.

for isolation and cultivation of Lactobacillus species