COLIFORM BROTH

Bacteriological Examination of water samples to determine its suitability for drinking and other domestic purpose has traditionally been done by the most probable number (MPN) procedures or the membrane filter (MF) technique. The presence of total coliforms, faecal coliforms or Escherichia coli is well recognized as an indication of unsafe or poor water quality for which corrective measures should be taken. Coliform Broth is recommended for isolation and cultivation of coliforms organisms from cream yogurt and raw milk. Coliforms that ferment lactose produce acid and gas. The acidity formed is indicated by a colour change of the medium from purple to yellow, indicated by the pH indicator dye bromo cresol purple.

for isolation and cultivation of coliform organisms from milk and milk products