SABOURAUD DEXTROSE AGAR MEDIUM W/ CHLORAMPHENICOL (as per IP)

Sabouraud Dextrose Agar Medium with Chloramphenicol is recommended for cultivation of yeasts and molds by Indian Pharmacopoeia. This medium was described originally by Sabouraud for the cultivation of fungi, particularly useful for the fungi associated with skin infections. The medium is often used with antibiotics such as Chloramphenicol for the isolation of pathogenic fungi from materials containing large numbers of fungi or bacteria. Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in the safety cabinet. Meat & Casein Peptone provide nitrogenous, carbonaceous compounds. Dextrose provides an energy source. Chloramphenicol inhibits a wide range of gram-positive and gram-negative bacteria making the medium selective for fungi. The low pH favors fungal growth and inhibits contaminating bacteria.

Sabouraud Dextrose Agar Medium is used in testing of food and dairy samples in controlled conditions when monitoring for fungal contamination. Sabouraud Dextrose Agar Medium allows for uniform isolation of yeasts and molds if good incubation conditions are followed. Use standard microbiological techniques to handle samples to avoid environmental contamination. In certain cases, growth of specific fungi may vary and additional confirmation methods may be required. The medium provides reliable performance when the medium is prepared, stored and used according to the laboratory instructions and the validated procedures.

for selective cultivation of yeasts and molds