V.C.N.T. SUPPLEMENT

Rehydrate the contents of 1 vial aseptically with 2 ml sterile distilled water. Mix well and aseptically add it to TM 933 –
THAYER MARTIN MEDIUM BASE for 440 ml of medium aseptically add 50ml sterile lysed blood and one vial of V.C.N.T
Supplement along with one vial of Vitamino Growth Supplement, 2% Haemoglobin Solution (250ml) can be used instead
of sterile lysed blood in 250ml of medium.
In TM 116 – GC AGAR BASE / TMV 116 – GC AGAR BASE (VEG.) for 250 ml of medium aseptically add 250 ml of 2%
Haemoglobin Solution and GC Supplement w/Antibiotics, one vial of Vitamino Growth Supplement w/ Antibiotics or Yeast
Autolysate Supplement. If desired V.C.N.T Supplement can be used along with GC Supplement w/ Antibiotics for
additional selectivity.
In TM 1105 – TRANSGROW MEDIUM BASE for 440 ml of medium aseptically add 50 ml of sterile 2% Haemoglobin Solution
and one vial of V.C.N.T Supplement along with one vial of Vitamino Growth Supplement.

For selective isolation of Neisseria gonorrhoeae and Neisseria meningitidis.