UREA INDOLE BROTH, MODIFIED (ISO 10273:2003)

Strains of Enterobacteria are associated with abscesses, pneumonia, meningitis, septicemia and infections of wounds, the urinary tract and the intestine. They are a major component of the normal intestinal flora of humans but are relatively uncommon at other body sites. Of clinically significant isolates, Enterobacteriaceae may account for 80% of gram-negative bacilli and 50% of all clinically significant isolates in clinical microbiology laboratories. This medium formulation is as per ISO and is recommended for the confirmation of Yersinia on the basis on urease reaction and indole reaction. Yersinia gives a positive urease reaction. Some biovars of Yersinia are indole positive while some give negative reaction. Urea Indole Medium is also used for the identification of Enterobacteria on the basis of urease and indole production and the trans-deamination of tryptophan. The results for urease production should be noted prior to indole reaction, as addition of Kovacs reagent, decolourizes the medium, due to drop in pH.

for con?rmation of Yersinia enterocolitica by urease and Indole test