OXIDASE DISCS

Product Code TBL 030

  • Description

    Certain bacteria posses either cytochrome oxidase or indophenol oxidase (an iron-containing haemoprotein), which
    catalyzes the transport of electrons from donor compounds (NADH) to electron acceptors (usually oxygen). In the
    oxidase test, a colourless dye such as N, N-dimethy-p-phenylenediamine serves as an artificial electron acceptor for the
    enzyme oxidase. The dye is oxidized to form indophenol blue, a coloured compound. The test is useful in the initial
    characterization of aerobic gram-negative bacteria of the genera Aeromonas, Plesiomonas, Pseudomonas,
    Campylobacter and Pasteurella. Oxidase discs are sterile filter paper discs impregnated with N, N-dimethyl-pphenylenediamine
    oxalate, ascorbic acid and a-naphthol. These discs overcome the neccessity of daily preparation of
    fresh reagent. Gordon and McLeod introduced oxidase test for identifying gonococci based upon the ability of certain
    bacteria to produce indophenol blue from the oxidation of dimethyl-p-phenylenediamine and a-naphthol. Gaby and
    Hadley introduced a more sensitive method by using N, N-dimethyl-p-phenylenediamine oxalate where all
    staphylococci were oxidase negative. In a positive reaction the enzyme cytochrome oxidase combines with N,Ndimethyl-
    p-phenylenediamine oxalate and a-naphthol to form the dye, indophenol blue.

  • Principle

    For detection of oxidase production by microorganisms.

  • Industry

    • Clinical Diagnostics
    • Cosmetics
    • Food & Beverages
    • Industrial & Lab Purposes
    • Pharmaceutical
    • Veterinary
  • Pack Size

    • 50 Discs/vl
  • Downloads

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