MUELLER HINTON AGAR PLATE W/ 5% SHEEP BLOOD

Mueller Hinton agar was originally developed as a simple, transparent agar medium for cultivation of Neisseria species. It is further enriched by the addition of sterile blood. Mueller Hinton Agar is now used as a test medium for antimicrobial susceptibility testing. This media is used as a base for preparing media containing blood and for selective media formulations in which different combinations of antimicrobial agents are used as additives. Mueller Hinton Agar with 5% Sheep Blood is recommended for disc diffusion susceptibility testing of Streptococcus pneumoniae with selected agents.
The Kirby-Bauer procedure is based on agar diffusion of antimicrobial substances impregnated on paper discs. This method employs disc with a single concentration of antimicrobial agent and the zone diameters observed are correlated with minimum inhibitory concentration (MIC) values.

For determination of susceptibility of Streptococcus species to antimicrobial agents