DIFFERENTIAL REINFORCED CLOSTRIDIAL BROTH BASE

Hirsch and Grinstead originally described Differential Reinforced Clostridial Medium to initiate the growth from small inoculum and get a higher Clostridial count. Later, Barnes and Ingram used the medium to develop vegetative cells in assays of Clostridium perfringens. This medium is developed for the isolation of sulphite-reducing Clostridia from food and for their enumeration in water by multiple tube method.

Differential Reinforced Clostridial Broth Base is used to determine the count of sulphite reducing bacteria by MPN technique. The medium contains peptone, beef extract, yeast extract, starch, and sodium acetate which supply the nutrients needed for bacterial metabolism. Glucose is the fermentable carbohydrate and provides a source of carbon and energy. L-cysteine hydrochloride is a reducing agent. Sodium sulphite and ferric citrate are added as indicators. Sulphite reducing clostridia produces sulphide from sulphite, which results in the formation of black coloured medium.

Differential Reinforced Clostridial Broth Base is suitable for routine analysis in laboratories for quantitative determination of sulphite reducing bacteria. Differential Reinforced Clostridial Broth Base gives uniform results when used as directed, and results in dependable detection. For accurate identification the results obtained can be confirmed by other microbiological procedures and routine testing methods.

for cultivation of Clostridia from water