C.L.E.D. AGAR (W/ BROMO THYMOL BLUE)(BROLACIN AGAR)

CLED Agar (Cystine Lactose- Electolyte- Deficient Agar) w/ Bromo Thymol Blue is a differential medium that was described by Mackey and Sandys to isolate coliforms from urine samples. On a solid medium, Sandys reported that swarming of Proteus species can be controlled by restricting the electrolytes. Later on Sandys medium was modified by Mackey and Sandys, by replacing mannitol with lactose and sucrose and elevating the concentration of agar and bromo thymol blue. This formulation was further modified by the same authors, called C.L.E.D. (Cystine-Lactose-Electrolyte-Deficient) by deleting the sucrose and including L-cystine for promoting the growth of cystine dependant dwarf colony coliforms.

for isolation and differentiation of urinary pathogen by lactose fermentation