Product Code TM 1934
Burkholderia cepacia is an important opportunistic pathogen and causes pulmonary infection among individuals with cystic ffibrosis (CF). The organism may lead to Burkholderia cepacia syndrome, a neutralizing pneumonia associated with fever that culminates in to a rapid and fatal clinical deterioration. B. cepacia is difficult to isolate on routinely used laboratory media like MacConkey Agar, since B. cepacia is a slow grower and therefore it is usually outgrown by the faster growing Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa . Burkholderia Cepacia Agar is based on PC medium, which was originally devised by Gilligan. This medium was found to be superior to MacConkey Agar for growth of B. cepacia. The medium is made selective for B. cepacia by the incorporation of bile salts, crystal violet and antibiotics. The antibiotics included are Polymyxin B, Gentamycin, Ticarcillin in the form of freeze dried supplement (FD). The antibiotics (FD) namely ticarcillin, polymixin B and gentamycin inhibit gramnegative bacteria. B. cepacia metabolizes pyruvate forming alkaline end products. These end products elevate the pH of the medium. The phenol red indicator changes colour from pink orange to pink red in alkaline pH.
for isolation of Burkholderia cepacia from the respiratory secretions of patients with cystic ?brosis and other non-clinical specimens