AGAR MEDIUM L (BRILLIANT GREEN, PHENOL RED, LACTOSE MONOHYDRATE,SUCROSE AGAR)

Brilliant Green, Phenol Red, Lactose Monohydrate, Sucrose Agar is used as a primary plating medium for isolation of Salmonella species was first described by Kristensen et al as medium for differentiation of paratyphoid B from other Gram negative enteric bacteria. It was further modified by Kauffmann for isolation of Salmonella from stool samples. This medium is employed in testing clinical specimens. Heavy inocula and heavily contaminated samples can be analyzed due to the outstanding selectivity of this medium. Brilliant Green Agar is used in the microbial limits test and with novobiocin for testing food samples.

for selective isolation of Salmonellae other than Salmonella Typhi from faeces, foods, dairy products