for identification and confirmation of microorganisms causing urinary tract infections. Urinary tract infections are bacterial infections affecting parts of urinary tract. The common symptoms of urinary tract infection are urgency and frequency of micturition, with associated discomfort or pain. The common condition is cystitis, due to infection of the bladder with a uropathogenic bacterium, which most frequently is Escherichia coli, but sometimes Staphylococcus saprophyticus or especially in hospital-acquired infections, Klebsiella species, Proteus mirabilis, other coliforms, Pseudomonas aeruginosa or Enterococcus faecalis. Chromogenic UTI Agar is formulated on basis of work carried out by Pezzlo Wilkie et al, Friedman et al, Murray et al, Soriano and Ponte and Merlino et al.
Peptone special provides nitrogenous, carbonaceous compounds and other essential growth nutrients while agar as a solidifying agent. UTI Agar contains two specific Chromogenic substrates which are cleaved by enzymes produced by Enterococcus spp., Escherichia coli and coliforms. In addition, it contains phenylalanine and tryptophan, which provide an indication of tryptophan deaminase activity, indicating the presence of Proteus spp., Morganella spp. and Providencia spp. One of the Chromogenic substrate is cleaved by ß- glucosidase possessed by Enterococci resulting in formation of blue colonies. E. coli produces pink colonies due to the enzyme ß-D-galactosidase that cleaves the other Chromogenic substrate. Further confirmation of E. coli can be done by performing the Indole test. Coliforms producepurple coloured colonies due to cleavage of both the Chromogenic substrate. Colonies of Proteus spp., Morganella spp. and Providencia spp. appear brown because of tryptophan deaminase activity