BUFFERED PEPTONE WATER (as per ISO)

Buffered Peptone Water is a non-selective pre-enrichment medium for the isolation of the Salmonella species from food and associated samples. The medium is designed to be used prior to selective enrichment. As Salmonella may be present in low numbers or sub-lethally injured, pre-enrichment allows cells time to repair and multiply before being introduced to selective culture, thereby improving the chances of recovery from sample. The composition and performance criteria of this medium are as per the applications laid down in ISO 6579-2002, whereas the quality control of Buffered Peptone Water includes testing in accordance with ISO 6579:2017 and ISO 11133-2014. The media contains Peptone as a source of carbon, nitrogen, vitamins and minerals. Sodium chloride maintains the osmotic balance and phosphates buffer the medium. The broth is rich in nutrients and produces high resuscitation rates for sub lethally injured bacteria and supports intense growth. The phosphate buffer system prevents bacterial damage due to changes in the pH of the medium.

Buffered Peptone Water is particularly useful in testing food and feed samples where recovery of stressed organisms is critical before selective procedures. Buffered Peptone Water ensures improved detection by allowing cells to stabilize under controlled conditions. For complete identification, further enrichment and plating on selective media are required following incubation.

for pre enrichment of injured Salmonella species prior to selective enrichment and isolation