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Which concentration of Agar / Gellan gum should be used?

Published: 15th Dec 2021, 17:53

Agar is the most commonly used gelling agent for preparing semisolid and solid plant tissue culture media. The agar concentrations commonly used in plant cell culture media fall in range between 0.5 and 1.0%; these concentrations give a firm gel at the typical pH of plant cell culture media. Another gelling agent commonly used for commercial as well as research purposes is Gellan gum. This product is synthetic and should be used at 1.5-2.5 g/litre, resulting in a clear gel which aids in detecting contamination.

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