Agar is the most commonly used gelling agent for preparing semisolid and solid plant tissue culture media. The agar concentrations commonly used in plant cell culture media fall in range between 0.5 and 1.0%; these concentrations give a firm gel at the typical pH of plant cell culture media. Another gelling agent commonly used for commercial as well as research purposes is Gellan gum. This product is synthetic and should be used at 1.5-2.5 g/litre, resulting in a clear gel which aids in detecting contamination.
In every microbiology laboratory, careful handling of microorganisms is a key priority. Even a minor error in the transfer of...
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In laboratory work, a minor error in handling liquids can compromise the accuracy of a whole experiment. Whether it is...
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In the microbiology laboratory, not all bacteria are the same. Some can be stained and seen easily while others are...
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In every microbiology laboratory, there are situations where selective isolation becomes critical. A specimen is received with a suspicion of...
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