Agar is the most commonly used gelling agent for preparing semisolid and solid plant tissue culture media. The agar concentrations commonly used in plant cell culture media fall in range between 0.5 and 1.0%; these concentrations give a firm gel at the typical pH of plant cell culture media. Another gelling agent commonly used for commercial as well as research purposes is Gellan gum. This product is synthetic and should be used at 1.5-2.5 g/litre, resulting in a clear gel which aids in detecting contamination.
Two bacterial colonies can appear nearly indistinguishable on an agar plate. Yet their metabolic activities can expose significant differences. A...
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A microbiologist studies a bacterial culture that looks no different from many others. Yet, its metabolic capabilities may be an...
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A small tube sits on a microbiologist’s bench, looking almost ordinary. Its orange medium waits for one biological event to...
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A microbiologist often looks beyond what appears on a culture plate. A tiny change in colour can reveal an important...
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A colony appearing on an agar plate may look like a simple result. Yet, that small colony reflects everything the...
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A microbiologist receives a mixed sample and prepares a fresh culture plate. The next question seems simple: which organism will...
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