
Agar is the most commonly used gelling agent for preparing semisolid and solid plant tissue culture media. The agar concentrations commonly used in plant cell culture media fall in range between 0.5 and 1.0%; these concentrations give a firm gel at the typical pH of plant cell culture media. Another gelling agent commonly used for commercial as well as research purposes is Gellan gum. This product is synthetic and should be used at 1.5-2.5 g/litre, resulting in a clear gel which aids in detecting contamination.
In the microbiology laboratory, not all bacteria are the same. Some can be stained and seen easily while others are...
Read More
During routine microbiology work, particularly while processing stool or food specimens, a common problem is the presence of mixed bacterial...
Read More
When working in a microbiology lab, not all microorganisms behave the same way. Some grow fast, some grow slowly, and...
Read More
In every microbiology laboratory, there are situations where selective isolation becomes critical. A specimen is received with a suspicion of...
Read More
In microbiology laboratories, there are moments when identifying enteric bacteria becomes critical. A sample may contain a mix of organisms,...
Read More
In microbiology, good results depend not only upon the techniques used in the laboratory, but also on the state of...
Read More